Dolomite Microfluidics

Dolomite Microfluidics are a one-stop-shop for droplet microfluidics. Watch the video to see our wide range of products and the applications that they benefit.

For more information, please visit: www.dolomite-microfluidics.com

Dolomite Droplet Microfluidics

Dolomite is the world leader in design and manufacture of innovative microfluidic products.

This video is a short summary of the application note describing the generation of agarose droplets at elevated temperature and subsequent cooling to form solid microparticles. The highest droplet generation rate is 2800 droplets per second with a droplet diameter of ~ 60µm. The system is based around a standard hydrophobic droplet junction chip.

To view the full application note titled Agarose Bead Synthesis please visit:
http://www.dolomite-microfluidics.co.uk/applications/application-notes#hydrogels

For more about hydrogels, please see 
http://www.dolomite-microfluidics.co.uk/en/applications/hydrogels

Agarose Particle Production

Using microfluidics, one or many cells can be isolated, in a highly reproducible way, in monodisperse water droplets in oil (i.e. in water-in-oil micro-emulsions). The aqueous droplets may contain buffer solutions, reagents and in some cases, magnetic beads. The oil prevents diffusion between the droplets, which can function as discrete ‘micro-reactors’. In some applications, the aqueous droplets may be within an oil shell, i.e. in water-in-oil-in water double emulsions.

This video briefly explains the two stage process of double emulsion production based on the application note which investigates a two-chip system for making 30µm double emulsion droplets, containing individual cells. 

To view the full application note visit: 
http://www.dolomite-microfluidics.co.uk/images/stories/PDFs/application_notes/cells-in-double-emulsions-for-FACS-sorting.pdf

For further information about cell encapsulation please visit:
http://www.dolomite-microfluidics.co.uk/en/applications/cell-encapsulation 

Cells in Double Emulsion Droplets

The µEncapsulator 1 System enables simple, quick and reliable encapsulation of single cells, DNA and/or functionalized beads in high precision, monodisperse picoliter droplets. The high-throughput system can encapsulate 300,000 cells into 3 million droplets in 15 minutes. 

Find out more: http://www.dolomite-microfluidics.com/webshop/uencapsulator1_system

µEncapsulator 1 - Single Cell Encapsulation

Fluidic Factory is the world's first commercially available 3D printer for quick and easy fabrication of fluidically sealed devices.

Design your own microfluidic chips, manifolds, connectors etc. or select from the design library and print for as little as $1 per chip using COC polymer (FDA approved, robust and translucent).

The intelligent printer ensures fluidic paths are sealed and features an upgradable print head, bed and software for future functionality.

Find out more: http://www.dolomite-microfluidics.com/webshop/fluidic_factory

Fluidic Factory 3D Printer for Fluidically Sealed Devices

Many biologically important events occur at the level of cells, so it has long been desired to analyze and follow tissues or cell populations at single cell resolution. For the first time, microfluidic droplet-based methods allow researchers to rapidly discover new cell types and follow the behavior of thousands of cell types. Using microfluidic droplets allows small samples to produce tens of thousands of single cell libraries on uniquely bar-coded beads in a short amount of time. The libraries are then ready for high-throughput sequencing.

For more information, please visit http://www.dolomite-microfluidics.com 

Dolomite Microfluidics

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