Phase Focus

Dr Rakesh Suman, a Phasefocus-sponsored Knowledge Transfer Partnership (KTP) Associate in the Bioscience Technology Facility, Department of Biology, University of York, shared the runners' up spot with his creation of Cellular Heaven and Hell, a time-lapse video obtained with the Phasefocus Virtual Lens VL20 microscope, which uses ptychography to produce label-free, high-contrast, quantitative phase images. The healthy human bladder epithelial cells in "heaven" were imaged under normal physiological conditions every 10 minutes and are played back at seven frames per second. The human gut epithelial cells in "hell" were treated with staurosporine to induce cell death, or apoptosis.

Cellular Heaven and Hell - ASCB Runner Up!

Thresholded timelapse video of A549 cells imaged using the Phasefocus Virtual Lens® (http://www.phasefocus.com) at 37°C for 20 hours, illustrating the quantitative nature of our images. Courtesy of Peter O'Toole, Joanne Marrison, Lotta Räty, Poppy Marriott and Ian Morrison, Imaging and Cytometry, Department of Biology, University of York, Hesligton, York, Y010 5DD, UK

Thresholded timelapse video of  label-free A549 cells imaged using the Phase Focus Virtual Lens®

Timelapse video of  label-free A549 cells imaged using the Phase Focus Virtual Lens® at 37°C for 20 hours, courtesy of Peter O’Toole, Joanne Marrison, Lotta Räty, Poppy Marriott and Ian Morrison, Imaging and Cytometry, Department of Biology, University of York, Hesligton, York, Y010 5DD, UK

Timelapse video of  label-free A549 cells imaged using the Phase Focus Virtual Lens®

Timelapse video of label-free  B104 Neurone cells imaged using the Phase Focus Virtual Lens® at 37°C for 20 hours, courtesy of Peter O’Toole, Joanne Marrison and Rakesh Suman, Imaging and Cytometry, Department of Biology, University of York, Hesligton, York, Y010 5DD, UK. 

Timelapse video of label-free  B104 Neurone cells imaged using the Phase Focus Virtual Lens®

Label-free timelapse video of human bladder epithelial cells, imaged using the Phase Focus Virtual Lens® every 10 minutes for 33 hours

Label-free timelapse video of human bladder epithelial cells, imaged using the Phase Focus Virtual Lens®

We are redefining microscopy using a new and unique imaging method, the Phase Focus Virtual Lens®.  The technology uses a phase retrieval algorithm to create high fidelity images from scattered waves, allowing us to accurately reconstruct properties of an object, adding a new quantitative dimension to imaging and microscopy. This technique completely eliminates limitations inherent in traditional optical lenses.

Phase Focus

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