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Learn how to visualize your proteins of interest with IHC on brain slice sections after electrophysiology

IHC for brain slice sections

Brain slice electrophysiology allows the study of a synapse or neural circuit in isolation from the rest of the brain, in controlled physiological conditions. This protocol describes the preparation of hippocampal slice and electrophysiology recording.

Brain slice electrophysiology

Detailed procedure explaining how to obtain a viable cell count from a hemocytometer

Counting cells using a hemocytometer

Cryopreservation is a method whereby cells are frozen, maintaining their viability, until they are defrosted months or years later. Cells are cryopreserved to minimize genetic change and avoid loss through contamination. It is best to cryopreserve cells when they are at their optimal rate of growth.

Cryopreservation of mammalian cell lines

Immunocytochemistry/immunofluorescence (ICC/IF) is a technique that uses fluorescent antibodies or dyes to detect target antigens within cells. ​This protocol describes the procedure for ICC/IF of suspension cells using cytocentrifugation

ICC for suspension cells using cytocentrifugation

The embedding of tissue into paraffin blocks supports its structure and allows very fine sections to be cut and mounted onto microscope slides. This video demonstrates the process of immunohistochemistry (IHC) where paraffin sections are labeled with a variety of antibodies and stains to reveal the molecular architecture of the tissue.

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