Real-time visual validation of treatments and conditions in IncuCyte™ Scratch Wound Migration and Invasion Assays. Differences in HT-1080 morphology and rate of wound closure between migrating and invading cell are observed. Rate of migration is greater than rate of invasion can also be observed. All 3 cell types migrate on collagen I coated wells. Highly invasive HT-1080 and MDA-MD-231 cell invade 3D collagen gel (1, 2 and 3 mg/ml). Relatively non-invasive MCF-7 cells do not invade 3D collagen gel under the condition of the assay.
Cell ScienceDrug DiscoveryImmunology