SpectraMax M5e Multi-Mode Microplate Reader

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Molecular Devices

The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5e Micoplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform. - UV-Visible Absorbance (Abs) - Fluorescence Intensity (FI) - Time-Resolved Fluorescence (TRF) including CisBio HTRF Certification - Fluorescence Polarization (FP) - Glow Luminescence (Lumi) The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed optical pathlength cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantitation. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5e Micoplate Reader optical design provides the highest level of flexibility. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Assays can be better optimized by scanning across a range of wavelengths in increments as small as 1 nm. Up to 4 wavelength pairs can be read in one protocol for endpoint and kinetic measurements, allowing for fast setup of FRET and TR-FRET assays, including CisBio's HTRF assay. For luminescence, the SpectraMax M5e Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. The SpectraMax M5e Microplate Reader is supplied with SoftMax Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one software.