Nanoparticle Tracking Analysis – A Novel Technique for Exosomes Research

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September 13, 2013

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  • The technique of Nanoparticle Tracking Analysis from NanoSight Ltd is a recently developed method which is being rapidly adopted for the analysis and counting of exosomes and microvesicles. Based on a unique microscopical laser illumination configuration, this technique allows biological nanoparticles in a sample to be simultaneously but individually visualised, sized (through video analysis of their Brownian motion) and counted in real time and in complex backgrounds. Furthermore, it is capable of detecting and analysing fluorescently labelled exosomes and microvesicles allowing for phenotyping of subpopulations of such structures in clinical samples. The technique will be described and selected examples will be shown from the 250+ papers describing the use of NTA in the routine analysis of biological samples of clinical significance in a wide range of applications concentrating on the use of exosomes and microvesicles in diagnosis and therapeutics.

    Flow Chemistry

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